Stable Cell Line Generation Protocol

The generation of stably transfected cell lines is critical for a wide range of applications. Protocol for generation of stable cell lines 2. Stable cell line generation is made possible by the use of positive selection markers such as g418 hygromycin b puromycin resistance etc.

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Protocol of stable cell line generation 1.

Stable cell line generation protocol.

Protocol of stable cell line generation. Unlike the short term protein expression observed using transient transfection approaches generating cell lines using lentiviral vectors enables long term protein expression studies. Stable expression of target gene in cell lines overcomes the low transfection. Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with the plasmid containing the gene of interest.

This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. It is applied to the production of recombinant proteins gene function studies as well as drug discovery assays. 1 applications types major challenges transfection methods selection marker methods and culture conditions of stably transfected cell lines. Stable cell line generation is made possible by the use of positive selection markers such as hygromycin g418 geneticin zeocin and blasticidin antibiotic resistance.

Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with.

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